Protein Verification

SMART™ Micro BCA Protein Assay Kit

● Description BCA assay variation method for 2~40 mg/ml small amount protein extraction §  A very sensitive reagent for dilute protein samples §  Linear and wide working range (BSA, 2 ~ 40 ug/ml) §  Compatible with most ionic and nonionic detergents. §  Less protein-protein variation that dye-binding methods §  Convenient use with Micro-plate(96 well plate). SMART™ micro BCA Protein Assay Kit is detergent-compatible  bicinchoninic acid formulation for the colorimetric detection and quantitation of total protein. An adaptation of the SMART™ BCA Protein Assay Kit (for Standard Assay), SMART™ micro BCA Protein Assay Kit (for Micro Assay) has been optimized for use with dilute protein samples (2-40 ug/ml). The purple-colored reaction product of this assay is formed by thechelation of two molecules of BCA with one cuprous ion. This water-soluble complex exhibits a strong absorbance at 562 nm that is nearly linear with increasing protein concentrations. The macromolecular structure of protein, the number of peptide bonds and the presence of four particular amino acids(cystein,cystine, tryptophan and tyrosine)are reported to be responsible for color formation with BCA. Studies with di-, tri- and tetrapeptides suggest that the extent of color formation caused by more than the mere sum of individual colorproducing functional groups. SMART™ micro BCA Protein Assay Kit (for Micro Assay) uses concentrated solutions and a protocol that utilizes an extended incubation time at an elevated temperature (60℃, Test Tube Procedure only). The result is an extremely sensitive colorimetric protein assay in a microplate or test tube assay format.