RT/RT- PCR PreMix

HiSenScript™ RH(-) One-Step RT-PCR PreMix Kit 

Description         The HiSenScript™ RH(-) One-Step RT-PCR Kit is designed for high sensitive and reproducible detection and analysis of low copies from either a viral RNA or RNA molecules by RT-PCR. Both reverse transcription and PCR are performed in a single tube using target gene specific primer/probe and template RNAs from either total RNA or mRNA. The reaction conditions forHiSenScript™ RH(-) One-Step RT-PCR Kit have been optimized to support a wide range of RT-PCR applications including real-time quantitative procedures. RT-PCR Enzyme mix is a mixture of engineered version of REV (Reticuloendotheliosis virus; REV) reverse transcriptase that reducedRNase H activity and recombinant DNA polymerase complexes with proprietary hot-start specific antibody that inhibits polymerase activity to synthesize DNA from an RNA template. HiSenScript™ RH(-) Reverse Transcriptase can synthesize cDNA at a temperature range of 40–50°C, providing increased specificity, more full-length product than other reverse transcriptase. i-StarMAX™ GH DNA polymerase activity is restored after the denaturation step in PCR cycling at 94°C, providing an automatic “hot start” in PCR for increased sensitivity, specificity, and yield. The 2X RT-PCR Reaction Solution included in consists of a proprietary buffer system that has been optimized for reverse transcription and PCR, Mg2+, dNTPs, tracking dye and stabilizers. The convenient 2X format allows you to add template and primer at any desired concentration.