RT Enzyme

M-MLV Reverse Transcriptase

● Description Reverse Transcriptase originated from Moloney murine leukemia virus for cDNA synthesis M-MLV Reverse Transcriptase is one of DNA polymerase  and used for the synthesis of  Single stranded RNA, DNA or complementary DNA strand from RNA:DNA hybrid like AMV RT. This is also commonly used for cDNA synthesis from RNA but has low endonuclease activity as well as RNase Hactivity comparing to AMV RT  § Source  Purified from Moloney murine leukemia virus  § Unit  Definition     One unit of the enzyme incorporates 1 nmol dTTP into acid-precipitable material in 10 min at 37℃, using poly(A); oligo dT as a template:primer  § Quality control      1) Endonuclease Activity : 1 mg of Type I supercoiled plasmid DNA is incubated with 500             units of enzyme in 1x reaction buffer for 1 hr at 37℃. The supercoiled DNA is visualized          on EtBr-stained agarose gel to verify the absence of nicking or cutting.      2) Nuclease Activity : 50 ng of radiolabeled DNA or RNA is incubated with 200 units of          enzyme in 1x reaction buffer for 1 hr at 37℃, resulting ing < 1% release for both DNase          and RNase.           §Physical purity  > 90% as judged by SDS-PAGE gel with coomassie blue staining  §General use   Use 1 ml of M-MLV RT in a 20 ml of reaction. http://www.amb.vn