Total RNA Prep

R&A-BLUE™ Total RNA Extraction Kit

● Description Solution type product which can extract high yield and purity of Total RNA from cell, tissue or plant, etc. and possible to store at Room Temperature. § Can be stored at room temperature for 1 year, and at 2-8°C for 2 years by oxidation control of      Phenol. § Excellent stability and easy to use with mild smell by no formation of ortho-chlorophenol. § Effectively control of gDNA inflow, which affect product oxidation in long-term storage, thru    antioxidant activity. § Excellent efficiency of phase separation on both small amount of samples and large amount of    samples. § Maximized yield by high dissociation-efficiency of solid tissue and cell wall.  Possible to extract RNA effectively from the sample which has metabolite like lipid and  polysaccharide.    R&A-BLUE™ Total RNA Extraction Kit is exemplary for quick, economical, and  efficient isolation of total RNA or the isolation of RNA from samples of human, animal, plant, bacterial and viral origin. A reagent of phenol and guanidine-salt inhibits RNase activity, and so the reagent maintains integrity of the RNA during sample homogenization or lysis. A biological sample is homogenized or lysed in R&A-BLUE™ and the homogenate is separated  into aqueous and organic phases by chloroform addition and centrifugation. RNA remains exclusively in the aqueous phase, DNA in the inter phase and proteins in the organic phase. RNA is precipitated from the aqueous phase by addition of 2-propanol, washed with ethanol and solubilized. R&A-BLUE™ Total RNA Extraction Kit performs well with large or small amount of tissue or cells and many samples can be simultaneously extracted. This is one of the most effective methods for isolating total RNA and can be completed in only 1 hour starting with fresh tissue or cells. The procedure is very effective for isolating RNA molecules of all types. The isolated RNA is intact with little or no contaminating genomic DNA. This RNA can be used for Northern blot, cDNAsynthesis, in vitro translation, cloning and RT-PCR.