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Agarose

● Description Easy-to-handle gels for PCR product separation and blotting; ≥ 1000 bp DNA fragments Agarose Solid 3:1 has enough strength and also maintains separation ability. It is optimal for southern blotting and northern blotting  because it is suitable for 50 bp ~ 1 Kb like short DNA/RNA separation. §  Gel strength (4%) ≥ 1,400g/cm2 §  Gelling Temp range (4%)  32.5℃ ~ 38℃  §  Melting Temp(4%) ≤ 90℃ §  DNase/RNase Non detected

Chemical & Other

● Description The essential product to remove RNase from lab ware and surface for RNA work §  Effective removal of RNase contamination caused by environment §  Safety and no effect on the follow-up test         No use detergent of high concentration or DEPC §  Ready to use  : Spray type §  Easy to use : Spray / Rinse or Wipe §  Easy to wipe plastic, glass and pipette which are difficult to sterilize §  Applicable for protection from RNase and DNA contamination RNase WiPER™ is used to remove RNase which affects on the test result from bench tops, instruments, pipettes, glass and plastic ware. It is easy to use, just spray and wipe on work surface, but effective to remove RNase contamination. As highly concentrated detergent or DEPC is not used, the product is safe for the experimenter. The strong acid is not used, either, it can guarantee the safety and does not have an effect on the follow-up experiment. This product is to make optimal condition for RNA work but not similar chemical with RNase inhibitor,  therefore, direct adding to reaction buffer is prohibited.

catalogue

Mycoplasma

DPBS. 20X without Ca, Mg

Cell culture

MTT Cell Proliferation Assay Kit

● Description Cell proliferation can be easily measured by MTT assay kit - Don’t use a radioactive isotope - Accurate and small quantity can measure - Ready-to-use reagent - Many samples can measure at the same time using ELISA reader - All cell type can use Adherent cell and suspension cell  MTT Cell Proliferation Assay Kit provides a simple method for determination of cell number using ELISA reader. Determination of cell growth rates is widely used in the testing of drug action, cytotoxic agents and screening other biologically active compounds. Several method can be used for such determinations, but indirect approaches using fluorescent or chromogenicindicators provide the most rapid and large scale assays among such procedures, the MTT assay is still among one of the most versatile and popular assays.   The absorbance of this colored solution can be quantified by measuring at a certain wavelength (usually between 550 and 600 nm) by a spectrophotometer. The absorption maximum is dependent on the solvent employed.

Transfection

iN-fect BOND Cell Adhesion Reagent

● Description Cell growth & Transfection efficiency, stronger than ever before!!! •   Polymer synthetic protein •   Enhancement of Cell growth, bonding and transfection efficiency •   Applicable to fibroblast and the most primary cells •   Ready-to-use products. iN-fect BOND™ Cell Adhesion Reagent has a special formulation of polymer synthetic protein that are mainly used to improve the adhesion of different cell types to the surface of dishes and wells of plates. iN-fect BOND™ Cell Adhesion Reagent can increase the survival rate of cell lines from 5% to 95% during the cell growth transfer process. Most of cell type is less affected byserum protein and shows better adhesion to the surfaces. Especially when serum-free or low-serum medium is used or when experiments such as transfection are implemented, the cultivation efficiency of individual cell lines can be improved.

Mutagenesis

Muta-Direct™ Site Directed Mutagenesis Kit

● Description  Research product for mutagenesis with 3 steps §  Easy use with the optimized protocol  §  Induction of point mutation with 3 steps in 2 days §  Convenience by using Muta-Direct™ and Mutazyme™ enzyme §  Various application for point mutation, deletion and insertion, etc. §  100% mutagenesis efficiency §  Reasonable price     Muta-Direct™ Site-Directed Mutagenesis Kit  is designed to induce mutagenesis at the specific point of genewhich was cloned on plasmid DNA. In theory, the efficiency of mutagenesis would be 100% and all experimental procedures are complete with simple and convenient method within 2 days. It dose not require special vector such as M13 or DNA methylation step for the point mutation. Muta-Direct™ Site-Directed Mutagenesis Kit requires no special skills and the optimized protocol is provided to induce mutation  with simple method .

Transformation

Rapid MacCellTM DH5α 

●Description

Cloning

pLUG-Prime® TA-Cloning Vector Kit + MacCell (JM109)

●Description

http://www.amb.vn

Master Mix Kit

RealMOD™ GH Green Real-time PCR Master Mix Kit (2X) 

Core Kit

RealMOD™ Real-time PCR core Kit

● Description Real time PCR Core Kit based on Gene specific Primer/probe §  Adjustable details for condition of Real-time PCR §  Product based on Gene specific primer & probe §  High sensitivity         Quantitative analysis for the template less than pg §  Dynamic range         Linear quantitative PCR from DNA template of 1ng-1fg without ROX adjustment §  Accurate detection of the template of dynamic range §  High reproducibility RealMODTM Real-time PCR core Kit is for Real-time PCR using labeled probe such as TaqMan probe assay and optimized Taq DNA polymerase and buffer, etc. are provided individually to adjust the details of PCR condition. It is optimized for TaqMan method using specific primer and probe and has high specificity and amplification efficiency. Also the sensitivity for TaqMan analysis is high and the range of reaction is dynamic. To use the product flexibly according to the experimental conditions, each contents are individually provided. Experimenter can set up the condition for the specific gene detection and the product includes i-StarTaq DNA Polymerase of ultrapure grade and Hot-start PCR is available.  The optimized buffer included can improve the sensitivity, specificity and amplification rate and the reaction range is dynamic, that is suitable for quantitative PCR. 10X Buffer(w/ or w/o MgCl2) , MgCl2 and dNTP, etc. are provided respectively.

http://www.amb.vn

Other

RT Enzyme

M-MLV Reverse Transcriptase

● Description Reverse Transcriptase originated from Moloney murine leukemia virus for cDNA synthesis M-MLV Reverse Transcriptase is one of DNA polymerase  and used for the synthesis of  Single stranded RNA, DNA or complementary DNA strand from RNA:DNA hybrid like AMV RT. This is also commonly used for cDNA synthesis from RNA but has low endonuclease activity as well as RNase Hactivity comparing to AMV RT  § Source  Purified from Moloney murine leukemia virus  § Unit  Definition     One unit of the enzyme incorporates 1 nmol dTTP into acid-precipitable material in 10 min at 37℃, using poly(A); oligo dT as a template:primer  § Quality control      1) Endonuclease Activity : 1 mg of Type I supercoiled plasmid DNA is incubated with 500             units of enzyme in 1x reaction buffer for 1 hr at 37℃. The supercoiled DNA is visualized          on EtBr-stained agarose gel to verify the absence of nicking or cutting.      2) Nuclease Activity : 50 ng of radiolabeled DNA or RNA is incubated with 200 units of          enzyme in 1x reaction buffer for 1 hr at 37℃, resulting ing < 1% release for both DNase          and RNase.           §Physical purity  > 90% as judged by SDS-PAGE gel with coomassie blue staining  §General use   Use 1 ml of M-MLV RT in a 20 ml of reaction. http://www.amb.vn

cDNA Synthesis

DNA-Eraser™ Genomic DNA Removal Kit 

RT/RT- PCR PreMix

HiSenScript™ RH(-) One-Step RT-PCR PreMix Kit 

Description         The HiSenScript™ RH(-) One-Step RT-PCR Kit is designed for high sensitive and reproducible detection and analysis of low copies from either a viral RNA or RNA molecules by RT-PCR. Both reverse transcription and PCR are performed in a single tube using target gene specific primer/probe and template RNAs from either total RNA or mRNA. The reaction conditions forHiSenScript™ RH(-) One-Step RT-PCR Kit have been optimized to support a wide range of RT-PCR applications including real-time quantitative procedures. RT-PCR Enzyme mix is a mixture of engineered version of REV (Reticuloendotheliosis virus; REV) reverse transcriptase that reducedRNase H activity and recombinant DNA polymerase complexes with proprietary hot-start specific antibody that inhibits polymerase activity to synthesize DNA from an RNA template. HiSenScript™ RH(-) Reverse Transcriptase can synthesize cDNA at a temperature range of 40–50°C, providing increased specificity, more full-length product than other reverse transcriptase. i-StarMAX™ GH DNA polymerase activity is restored after the denaturation step in PCR cycling at 94°C, providing an automatic “hot start” in PCR for increased sensitivity, specificity, and yield. The 2X RT-PCR Reaction Solution included in consists of a proprietary buffer system that has been optimized for reverse transcription and PCR, Mg2+, dNTPs, tracking dye and stabilizers. The convenient 2X format allows you to add template and primer at any desired concentration.

Buffer

i-StarMAX II 10x PCR Buffer

● Description 10x PCR Buffer included in i-StarMAXTM II DNA Polymerase is for the optimal PCR over  20Kb template. In case of using more amount of buffer than suggested amount in the protocol,  the separate purchase is available. The hot start function of  i-StarMAXTM II DNA Polymerase significantly reduces non-specific reaction and its proofreading function reduces error rate at the long PCR.

DNA Marker

SiZer™-100 DNA Marker Solution

● Description Ideal DNA marker for the size of 11 linear DNA fragments from 100 to 1,500 base pairs. §  Ideal item for sizing of 11 linear DNA fragments from 100 to 1,500 base pairs §  Ready-to-use §  High visibility/ resolution §  Use with RedSafe™ Nucleic Acid Staining Solution(Cat. No. 21141), Ethidium bromide and any other DNA staining SizerTM -100 DNA Marker Solution is recommended for sizing of the fragments DNA from 100 to 1,500 base pairs on nucleic acid electrophoresis.  The ladder consists of 11 DNA fragments, 100/200/300/400/500/600/700/800/900/1,000 /1,500 base pairs. Each band except 500bp band consists of concentration of 40 ng per 5 μl and 500bp are 100ng to distinguish the size. The ladder is supplied with loading buffer for ready to use. It can be used with RedSafe™ Nucleic Acid Staining Solution(Cat. No. 21141),Ethidium bromide and any other DNA  staining.

PCR other

6X Loading Dye Solution (XB)

PCR PreMix

Maxime™ PCR PreMix Kit (i-StarTaq™ GH) 

Description Hot start PCR is commonly used to enhance the specificity and sensitivity of PCR. Hot start PCR technique was developed as a method to minimize the deleterious effects of mispriming at lower temperatures during PCR. In a PCR reaction, even short incubations at temperatures below the optimum annealing temperature for a particular set of primers can result in mispriming, elongation and the subsequent formation of spurious bands. However, manual hot start PCR is inconvenient, time-consuming, and incurs a risk of cross contamination.  Maxime PCR PreMix Kit(i-StarTaq™GH) is the product what is mixed every component: i-StarTaq™GH DNA Polymerase, dNTP mixture, reaction buffer, and so all-in-one tube for 1 rxn of PCR. This is the product that can get the best result with the most convenience system. The first reason is that it has every components for PCR, so we can do PCR just add a template DNA, primer set, and D.W.. The second reason is that it has gel loading buffer to do electorphoresis, so we can do gel loading without any treatment. In addition, each batches are checked by a thorough QC., so its reappearance is high. It is suitable for various sample’s experience by fast and simple using method. Maxime PCR PreMix Kit(i-StarTaq™GH) using i-StarTaq™GH DNA Polymerase, it has been proven to prevent generation of nonspecific amplification products and primer-dimer artifacts that means more definitive PCR results can often be obtained in cases where generation of nonspecific amplification products is a problem, has convenience as well as high specificity and guarantees the reliable result and the reproducibility for various samples.